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B-h4-1BB/hCD27 mice

C57BL/6-Tnfrsf9tm1(TNFRSF9)Cd27tm1(CD27)/Bcgen • 121162

B-h4-1BB/hCD27 mice

Product nameB-h4-1BB/hCD27 mice
Catalog number121162
Strain nameC57BL/6-Tnfrsf9tm1(TNFRSF9)Cd27tm1(CD27)/Bcgen
Strain backgroundC57BL/6
NCBI gene ID3604,939 (Human)
AliasesILA; 4-1BB; CD137; CDw137; IMD109; T14; S152; Tp55; TNFRSF7; S152. LPFS2

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  • Targeting strategy
  • Phenotypic analysis

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    发表文章

      Targeting strategy

      Gene targeting strategy for B-h4-1BB/hCD27 mice. The exon 2-7 of mouse 4-1bb gene that encodes the extracellular domain was replaced by human 4-1BB counterpart gene in B-h4-1BB/hCD27 mice. The exons 1-5 of mouse Cd27 gene that encode the extracellular domain were replaced by human CD27 exons 1-5 in B-h4-1BB/hCD27 mice.

      Protein expression analysis
      Strain specific 4-1BB and CD27 expression analysis in homozygous B-h4-1BB/hCD27 mice by flow cytometry. Splenocytes were collected from wild-type mice (+/+) and homozygous B-h4-1BB/hCD27 mice (H/H) stimulated with anti-CD3ε in vivo (7.5 μg/mice for 24 hours, i.p.), and analyzed by flow cytometry with anti-4-1BB antibody and anti-CD27 antibody. Human 4-1BB and CD27 were exclusively detectable in homozygous B-h4-1BB/hCD27 mice (H/H) but not in wild-type mice. Mouse 4-1BB were detectable in wild-type mice. Mouse CD27 can detectable both in wild-type mice and B-h4-1BB/hCD27 mice (H/H) for anti-mCD27 antibody can binding with human and mouse CD27.
      * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-h4-1BB/hCD27 mice] (Cat# 121162) was purchased from Biocytogen.