mRNA expression analysis
Strain specific analysis of CCL22 gene expression in wild type (WT) mice and B-hCCL22 mice by RT-PCR.Mouse Ccl22 mRNA was detectable only in thymus of WT mice (+/+). Human CCL22 mRNA was detectable only in homozygous B-hCCL22 mice (H/H) but not in WT mice (+/+).
Protein expression analysis
Strain specific CCL22 expression analysis in wild type (WT) mice and B-hCCL22 mice by ELISA.Thymus homogenates were collected from WT mice (+/+) and homozygous B-hCCL22 mice (H/H), and analyzed by ELISA with species-specific CCL22 ELISA kit. Mouse CCL22 was detectable in WT mice. Human CCL22 was exclusively detectable in homozygous B-hCCL22 mice (H/H) but not WT mice (+/+) . Values are expressed as mean ± SEM. ND: not detectable.
* When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hCCL22 mice] (Cat# 110817) was purchased from Biocytogen.
