C57BL/6-Cd28tm1(CD28)Bcgen Cd276tm1(CD276)Bcgen/Bcgen • 121251
| Product name | B-hCD28/hB7-H3 mice |
|---|---|
| Catalog number | 121251 |
| Strain name | C57BL/6-Cd28tm1(CD28)Bcgen Cd276tm1(CD276)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 80381,940 (Human) |
| Aliases | B7H3; B7-H3; B7RP-2; 4Ig-B7-H3; Tp44; IMD123 |
Gene targeting strategy for B-hCD28/hB7-H3 mice.
The exons 2-3 of mouse Cd28 gene that encode the extracellular domain were replaced by human CD28 exons 2-3 in B-hCD28/hB7-H3 mice. The exons 3~4 of mouse Cd276 gene that encode the extracellular domain were replaced by human CD276 exons 3~6 in B-hCD28/hB7-H3 mice.
Strain specific CD28 expression analysis in homozygous B-hCD28/hB7-H3 mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD28/hB7-H3 mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD28 antibody. Mouse CD28 was detectable in WT mice (+/+). Human CD28 was exclusively detectable in homozygous B-hCD28/hB7-H3 mice (H/H;H/H) but not in WT mice (+/+).
Strain specific B7-H3 expression analysis in homozygous B-hCD28/hB7-H3 mice by western blot.
Epididymis were collected from wild type (WT) mice (+/+) and homozygous B-hCD28/hB7-H3 mice (H/H;H/H), and analyzed by western blot with anti-B7-H3 antibody. B7-H3 was detectable in WT mice (+/+) and homozygous B-hCD28/hB7-H3 mice (H/H;H/H) due to the cross-reactivity of antibodies.
