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    B-hDDR1 mice

    C57BL/6-Ddr1tm1(DDR1)Bcgen/Bcgen • 111121

    B-hDDR1 mice

    Product nameB-hDDR1 mice
    Catalog number111121
    Strain nameC57BL/6-Ddr1tm1(DDR1)Bcgen/Bcgen
    Strain backgroundC57BL/6N
    NCBI gene ID780 (Human)
    AliasesCAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A

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    • Targeting strategy
    • Phenotypic analysis

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      发表文章

        Targeting strategy

        Gene targeting strategy for B-hDDR1 mice. The exons 3-12 of mouse Ddr1 gene that encode the extracellular sequences were replaced by human DDR1 exons 2-11 in B-hDDR1 mice.

        mRNA expression analysis
        Strain specific analysis of DDR1 gene expression in wild-type C57BL/6 mice and B-hDDR1 mice by RT-PCR. Mouse Ddr1 mRNA was detectable only in brain of wild-type C57BL/6 mice (+/+). Human DDR1 mRNA was detectable only in homozygous B-hDDR1 mice (H/H), but not in wild-type mice.
        Protein expression analysis

        Strain specific DDR1 expression analysis in homozygous B-hDDR1 mice by western blot. Brain collected from wild-type C57BL/6 mice (+/+) and homozygous B-hDDR1 mice (H/H), and analyzed by western blot with DDR1 antibodies. Human DDR1 was exclusively detectable in homozygous B-hDDR1 but not in wild-type mice. Mouse DDR1 was detectable in wild-type mice and homozygous B-hDDR1. The first DDR1 antibody is a human-specific antibody and the other is a human-mouse cross-recognition antibody.

        * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hDDR1 mice] (Cat# 111121) was purchased from Biocytogen.