• 德晋贵宾厅

    B-hDR4 mice

    C57BL/6-Igs2tm2(DR4)Bcgen/Bcgen • 111986

    B-hDR4 mice

    Product nameB-hDR4 mice
    Catalog number111986
    Strain nameC57BL/6-Igs2tm2(DR4)Bcgen/Bcgen
    Strain backgroundC57BL/6
    NCBI gene ID8797 (Human)
    AliasesDR4; APO2; CD261; TRAILR1; TRAILR-1

    在此页面上

    • Description
    • Targeting strategy
    • Phenotypic analysis

    海报

    查看全部

      发表文章

        相关产品

        查看全部
        Description

        DR4 is 56 kD member 10A of the TNFR superfamily (TNFRSF10A), also known as TRAIL-R1, Apo-2, and CD261.

        It is expressed at low levels by activated T cells and some tumors. After TRAIL engagement, DR4 (TRAIL-R1), through activation of NF-κB, induces apoptosis in the TRAIL ligated cell.

        The whole human DR4 genome was inserted at Hipp11 (H11) locus in B-hDR4 mice.

        Human DR4 was detectable in homozygous B-hDR4 mice stimulated with anti-CD3ε in vivo but not in wild-type mice.

        Targeting strategy

        Gene targeting strategy for B-hDR4 mice. The whole human DR4 genome was inserted at Hipp11 (H11) locus in B-hDR4 mice.

        Protein expression analysis
        Strain specific DR4 expression analysis in homozygous B-hDR4 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hDR4 mice (H/H) stimulated with or without anti-mCD3ε in vivo (7.5 μg/mice, stimulation for 24 hours, i.p.), and analyzed by flow cytometry with species-specific anti-DR4 antibody. Human DR4 was detectable in homozygous-stimulated B-hDR4 mice but not in wild-type mice.
        * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hDR4 mice] (Cat# 111986) was purchased from Biocytogen.