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    B-hFGFRL1 mice

    C57BL/6N-Fgfrl1tm2(FGFRL1)Bcgen/Bcgen • 112585

    B-hFGFRL1 mice

    Product nameB-hFGFRL1 mice
    Catalog number112585
    Strain nameC57BL/6N-Fgfrl1tm2(FGFRL1)Bcgen/Bcgen
    Strain backgroundC57BL/6N
    NCBI gene ID53834 (Human)
    AliasesFHFR; FGFR5; FGFR-5

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    • Targeting strategy
    • Phenotypic analysis

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      发表文章

        Targeting strategy

        Gene targeting strategy for B-hFGFRL1 mice. The exons 3-7 of mouse Fgfrl1 gene that encode the extracellular domain were replaced by human FGFRL1 exons 3-7 in B-hFGFRL1 mice.

        Protein expression analysis
        Western blot analysis of FGFRL1 protein expression in homozygous B-hFGFRL1 mice. Various tissue lysates were collected from C57BL/6 wildtype mice (+/+) and homozygous B-hFGFRL1 mice (H/H), and then analyzed by western blot with species-specific anti-FGFRL1 antibody. 40 μg total proteins were loaded for western blotting analysis. FGFRL1 was detected in lung.
        mRNA expression analysis

        Strain specific analysis of FGFRL1 mRNA expression in wild-type C57BL/6 mice and B-hFGFRL1 mice by RT-PCR. Kidney RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hFGFRL1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human FGFRL1 primers. Mouse Fgfrl1 mRNA was detectable only in wild-type C57BL/6 mice. Human FGFRL1 mRNA was detectable only in homozygous B-hFGFRL1 mice but not in wild-type mice.

        * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hFGFRL1 mice] (Cat# 112585) was purchased from Biocytogen.