Targeting strategy
Gene targeting strategy for B-hHCAR1 mice. The exon 1 of mouse Hcar1 gene that encodes the full-length protein was replaced by human HCAR1 exon 1 in B-hHCAR1 mice.
mRNA expression analysis
Strain specific analysis of HCAR1 gene expression in wild-type C57BL/6 mice and B-hHCAR1 mice by RT-PCR. Mouse Hcar1 mRNA was detectable only in BAT (brown adipose tissue) of wild-type C57BL/6 mice (+/+). Human HCAR1 mRNA was detectable only in homozygous B-hHCAR1 mice (H/H), but not in wild-type mice.
Protein expression analysis
Strain specific HCAR1 expression analysis in homozygous B-hHCAR1 mice by flow cytometry. BAT (brown adipose tissue) was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hHCAR1 mice (H/H), and analyzed by western blot with anti-HCAR1 antibody. HCAR1 was both detectable in wild-type mice and homozygous B-hHCAR1 mice (H/H) due to the cross-reactivity of antibodies.
* When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hHCAR1 mice] (Cat# 111919) was purchased from Biocytogen.
