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B-hIL36A mice

C57BL/6N-Il36atm1(IL36A)Bcgen/Bcgen • 110092

B-hIL36A mice

Product nameB-hIL36A mice
Catalog number110092
Strain nameC57BL/6N-Il36atm1(IL36A)Bcgen/Bcgen
Strain backgroundC57BL/6N
NCBI gene ID27179 (Human)
AliasesFIL1; FIL1E; IL1F6; IL-1F6; IL1(EPSILON); FIL1(EPSILON)

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  • Targeting strategy
  • Phenotypic analysis
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    发表文章

      Targeting strategy

      Gene targeting strategy for B-hIL36A mice.

      The exon 2-5 of mouse Il36a gene that encodes the full-length protein were replaced by human IL36A exon 1-4 in B-hIL36A mice.

      mRNA expression analysis

      Strain specific analysis of IL36A gene expression in wild type (WT) mice and B-hIL36A mice by RT-PCR. Mouse Il36a mRNA was detectable only in stomach of WT mice (+/+). Human IL36A mRNA was detectable only in homozygous B-hIL36A mice (H/H) but not in WT mice (+/+).

      Protein expression analysis
      Strain specific IL36A expression analysis in homozygous B-hIL36A mice by western blot . Skin were collected from wild type (WT) mice (+/+) and homozygous B-hIL36A mice (H/H), and analyzed by western blot with anti-IL36A antibody. Mouse IL36A was detectable in WT mice (+/+) due to the cross-reactivity of antibodies. Human IL36A was detectable in homozygous B-hIL36A mice (H/H) but not in WT mice (+/+).
      Product list
      * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hIL36A mice] (Cat# 110092) was purchased from Biocytogen.