德晋贵宾厅

    B-hIL9R mice

    C57BL/6-Il9rtm1(IL9R)Bcgen/Bcgen • 110761

    B-hIL9R mice

    Product nameB-hIL9R mice
    Catalog number110761
    Strain nameC57BL/6-Il9rtm1(IL9R)Bcgen/Bcgen
    Strain backgroundC57BL/6
    NCBI gene ID3581 (Human)
    AliasesCD129; IL-9R

    在此页面上

    • Targeting strategy
    • Phenotypic analysis

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      发表文章

        Targeting strategy

        Gene targeting strategy for B-hIL9R mice. The exons 2-7 of mouse Il9r gene that encode the extracellular coding sequence were replaced by human IL9R exons 2-7 in B-hIL9R mice.

        mRNA expression analysis
        Strain specific analysis of IL9R gene expression in B-hIL9R mice by RT-PCR. Mouse Il9 was detectable in splenocytes of wild-type mice (+/+). Human IL9R was detectable only in homozygous B-hIL9R mice but not in wild-type mice.
        Protein expression analysis

        Strain specific IL9R expression analysis in homozygous B-hIL9R mice by flow cytometry. Splenocytes were collected from wild-type mice (+/+) and homozygous B-hIL9R mice (H/H) stimulated with anti-CD40 in vitro (10 μg/mL), and analyzed by flow cytometry with species-specific anti-IL9R antibody. Mouse IL9R was detectable in wild-type mice. Human IL9R was exclusively detectable in homozygous B-hIL9R mice but not in wild-type mice.

        * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hIL9R mice] (Cat# 110761) was purchased from Biocytogen.