C57BL/6N-Muc17tm1(MUC17)Bcgen/Bcgen • 111292
| Product name | B-hMUC17 mice |
|---|---|
| Catalog number | 111292 |
| Strain name | C57BL/6N-Muc17tm1(MUC17)Bcgen/Bcgen |
| Strain background | C57BL/6N |
| NCBI gene ID | 140453 (Human) |
| Aliases | MUC3; MUC-3; MUC-17 |
Gene targeting strategy for B-hMUC17 mice. The exons 5-10 of mouse Muc17 gene that encode the SEA domain and part of the extracellular domain were replaced by human MUC17 exons 5-10 in B-hMUC17 mice.
Strain specific analysis of MUC17 gene expression in wild type (WT) mice and B-hMUC17 mice by RT-PCR. Mouse Muc17 mRNA was detectable only in small intestines of WT mice (+/+). Human MUC17 mRNA was detectable only in homozygous B-hMUC17 mice (H/H) but not in WT mice (+/+).
Strain specific MUC17 expression analysis in homozygous B-hMUC17 mice by flow cytometry. Single-cell suspension was obtained from intestinal tissue homogenates of wild type (WT) mice (+/+) and homozygous B-hMUC17 mice (H/H), and analyzed by flow cytometry with anti-human MUC17 antibody. Human MUC17 was exclusively detectable in homozygous B-hMUC17 mice (H/H) but not in WT mice (+/+).
