C57BL/6-Ntrk2tm1(NTRK2)Bcgen/Bcgen • 111070
| Product name | B-hNTRK2 mice |
|---|---|
| Catalog number | 111070 |
| Strain name | C57BL/6-Ntrk2tm1(NTRK2)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 4915 (Human) |
| Aliases | OBHD; TRKB; DEE58; trk-B; EIEE58; GP145-TrkB |
Gene targeting strategy for B-hNTRK2 mice. The exons 2-12 of mouse Ntrk2 gene that encode the extracellular and transmembrane domains were replaced by human NTRK2 exons 4-14 in B-hNTRK2 mice.
Western blot analysis of NTRK2 protein expression in homozygous B-hNTRK2 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hNTRK2 mice (H/H), and then analyzed by western blot with anti-NTRK2 antibody. 40 μg total proteins were loaded for western analysis. Human NTRK2 was detected in brain of B-hNTRK2 mice. Mouse NTRK2 was detected in brain of wild-type C57BL/6 mice.
Strain specific analysis of NTRK2 mRNA expression in wild-type C57BL/6 mice and B-hNTRK2 mice by RT-PCR. Brain RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hNTRK2 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human NTRK2 primers. Mouse Ntrk2 mRNA was detectable only in wild-type C57BL/6 mice. Human NTRK2 mRNA was detectable only in homozygous B-hNTRK2 mice but not in wild-type mice.
