C57BL/6-Cd274tm1(CD274)Bcgen Cd27tm1(CD27)Bcgen/Bcgen • 120537
| Product name | B-hPD-L1/hCD27 mice |
|---|---|
| Catalog number | 120537 |
| Strain name | C57BL/6-Cd274tm1(CD274)Bcgen Cd27tm1(CD27)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 29126,939 (Human) |
| Aliases | B7-H; B7H1; PDL1; PD-L1; ADMIO5; hPD-L1; PDCD1L1; PDCD1LG1; T14; S152; Tp55; TNFRSF7; S152. LPFS2 |
Strain specific PD-L1 expression analysis in homozygous B-hPD-L1/hCD27 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hPD-L1/hCD27 mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-PD-L1 antibody. Mouse PD-L1 was exclusively detectable in WT mice. Human PD-L1 were exclusively detectable in B-hPD-L1/hCD27 but not WT mice.
Strain specific CD27 expression analysis in homozygous B-hPD-L1/hCD27 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hPD-L1/hCD27 mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-CD27 antibody. Mouse CD27 was detectable in WT and B-hPD-L1/hCD27 mice. Human CD27 were exclusively detectable in B-hPD-L1/hCD27 but not WT mice. This might result from the cross-recognition of hCD27 by anti-mCD27 antibodies.
