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    B-NDG hGPC3 mice

    NOD.CB17-Prkdcscid Il2rgtm1Bcgen Gpc3tm1(GPC3)Bcgen/Bcgen • 113231

    B-NDG hGPC3 mice

    Product nameB-NDG hGPC3 mice
    Catalog number113231
    Strain nameNOD.CB17-Prkdcscid Il2rgtm1Bcgen Gpc3tm1(GPC3)Bcgen/Bcgen
    Strain backgroundNOD
    NCBI gene ID14734,16186,19090 (Human)
    AliasesOCI-5; gc; p64; [g]c; CD132; gamma(c); p460; scid; slip; DNAPK; DNPK1; HYRC1; XRCC7; dxnph; DOXNPH; DNAPDcs; DNA-PKcs

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    • Description
    • Targeting strategy
    • Phenotypic analysis
    • Physiological data

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      发表文章

        Description
        • GPC3 is a member of the heparan sulfate proteoglycan family anchored to the cell membrane. GPC3 activates the classical Wnt/β-catenin pathway in hepatocellular carcinoma to manifest an oncogene role.
        • A chimeric CDS that encodes human GPC3 extracellular domain, followed by mouse 3’UTR-STOP is inserted right after mouse Gpc3 signal peptide to replace partial exon 3 of mouse Gpc3 gene. The chimeric GPC3 protein expression will be driven by endogenous mouse Gpc3 promoter, while mouse Gpc3 gene transcription and translation will be disrupted.
        • Mouse Gpc3 mRNA was only detectable in wild-type B-NDG mice. Human GPC3 mRNA was only detectable in homozygous B-NDG hGPC3 mice.
        • Application: This product is used for validation of the efficacy of different antibody drugs and preclinical drug evaluation.
        Targeting strategy

        Gene targeting strategy for B-NDG hGPC3 mice. A chimeric CDS that encodes human GPC3 extracellular domain, followed by mouse 3’UTR-STOP is inserted right after mouse Gpc3 signal peptide to replace partial exon 3 of mouse Gpc3 gene. The chimeric GPC3 protein expression will be driven by endogenous mouse Gpc3 promoter, while mouse Gpc3 gene transcription and translation will be disrupted.

        mRNA expression analysis in humanized B-NDG hGPC3 mice

        Species specific analysis of GPC3 gene expression in wild-type B-NDG mice and homozygous humanized B-NDG hGPC3 mice by RT-PCR. Kidney was collected from wild-type B-NDG mice (+/+) and homozygous B-NDG hGPC3 mice (H/H). Mouse Gpc3 mRNA was detectable in wild-type B-NDG mice but not in homozygous B-NDG hGPC3 mice. Human GPC3 mRNA was detectable in homozygous B-NDG hGPC3 mice but not in wild-type B-NDG mice.

        IHC analysis of GPC3 expression

        Immunohistochemical (IHC) analysis of GPC3 protein expression in wild-type B-NDG mice and B-NDG hGPC3 mice. The liver and fetal liver were collected from B-NDG mice (+/+) and homozygous B-NDG hGPC3 mice (H/H) and analyzed by IHC with anti-GPC3 antibody (GC33-hIgG1 analog (in-house). Human GPC3 was detectable in fetal liver of homozygous B-NDG hGPC3 mice. The arrow indicates tissue cells with positive GPC3 staining (brown).

        * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-NDG hGPC3 mice] (Cat# 113231) was purchased from Biocytogen.