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    B-Bcrp KO rats

    SD-Abcg2tm1Bcgen/Bcgen • 113096

    B-Bcrp KO rats

    Product nameB-Bcrp KO rats
    Catalog number113096
    Strain nameSD-Abcg2tm1Bcgen/Bcgen
    Strain backgroundSD
    NCBI gene ID26357 (Mouse)
    AliasesMXR; ABCP; BCRP; MXR1; ABC15; Bcrp1

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    • Description
    • Targeting strategy
    • Phenotypic analysis

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      发表文章

        Description
        • BCRP consists of 655 amino acids and possesses six transmembrane helices and one ATP­ binding site. Because BCRP is a half­-transporter, current evidence suggests that homodimerization or multimerization is required for transporter activity as illustrated.
        • The exons 2~16 of ratBcrpgene were knocked out in B-Bcrp KO rats, resulting in a disruption of the Bcrp gene.
        • Bcrp mRNA and protein were detectable only in wild-type SD rats, but not in B-Bcrp KO rats.

        Suggestion:

        • Applicable for investigating ATP-powered transport proteins in physiological drug distribution.
        Targeting strategy

        Gene targeting strategy for B-Bcrp KO rats.The exons 2~16 of mouseBcrpgene were knocked out in B-Bcrp KO rats, resulting in a disruption of theBcrpgene.

        mRNA expression analysis in B-Bcrp KO rats

        BcrpmRNA expression in wild-type SD rats and B-Bcrp KO rats by RT-PCR. Kidney, brain and liver RNA were isolated from wild-type SD rats (+/+) and B-Bcrp KO rats (-/-), then cDNA libraries were synthesized by reverse transcription, followed by PCR with ratBcrpprimers. RatBcrpmRNA was detectable only in wild-type SD rats, but not in B-Bcrp KO rats.

        * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-Bcrp KO rats] (Cat# 113096) was purchased from Biocytogen.