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• The IL-10/IL-10R signaling pathway is a crucial anti-inflammatory and immunoregulatory pathway in the immune system. Acting on both innate and adaptive immune cells, it exhibits broad immunomodulatory activity by inhibiting the proliferation, cytokine secretion, and co-stimulatory molecule expression of pro-inflammatory immune cells. It plays a key role in regulating intestinal mucosal homeostasis.
• Gene editing strategy: The exons 1-5 of mouse Il10 gene were knocked out in B-Il10 KO mice(C), resulting in a disruption of the Il10 gene.
• Mouse Il10 mRNA was only detectable in wild-type BALB/cCrSlcNifdc mice, but not in homozygous B-Il10 KO mice(C). B-IL10 KO mice(C) were more susceptible to DSS than wild-type BALB/cCrSlcNifdc mice.
• This product is used for the pharmacodynamics evaluation of autoimmune diseases such as inflammatory bowel disease.

Gene targeting strategy for B-Il10 KO mice(C). The exons 1-5 of mouse Il10 gene were knocked out in B-Il10 KO mice(C), resulting in a disruption of the Il10 gene.

Species specific analysis of Il10 gene expression in wild-type BALB/cCrSlcNifdc mice and homozygous B-Il10 KO mice(C) by RT-PCR. Spleen was collected from wild-type BALB/cCrSlcNifdc mice (+/+) and homozygous B-Il10 KO mice(C) (-/-). Mouse Il10 mRNA was only detectable in wild-type BALB/cCrSlcNifdc mice, but not in homozygous B-Il10 KO mice(C).

Wild-type BALB/cCrSlcNifdc mice and B-IL10 KO mice(C) were administered DSS in drinking water for 8 consecutive days. The normal controls were administered drinking water, the treatment groups were administered 4% DSS solution. (A) Body weight change. (B) DAI score. (C) Colon index. (D) Colon photo. Values are expressed as mean ± SEM. Significance was determined by two-way ANOVA test.  *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Wild-type BALB/cCrSlcNifdc mice and B-IL10 KO mice(C) were administered DSS in drinking water for 8 consecutive days. The normal controls were administered drinking water, the treatment groups were administered 4% DSS solution. (A) H&E staining of colon tissue. (B) Pathological score. B-IL10 KO mice(C) were more susceptible to DSS than wild-type BALB/cCrSlcNifdc mice. Values are expressed as mean ± SEM. Significance was determined by two-way ANOVA test.  *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Wild-type BALB/cCrSlcNifdc mice and B-IL10 KO mice(C) were administered DSS in drinking water for 8 consecutive days. The normal controls were administered drinking water, the treatment groups were administered 4% DSS solution. On day 8, the mice were sacrificed, the serum was collected for IL-6 analysis. B-IL10 KO mice(C) were more susceptible to DSS than wild-type BALB/cCrSlcNifdc mice. Values are expressed as mean ± SEM. Significance was determined by two-way ANOVA test.  *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.