C57BL/6-Cd80tm1(CD80)Bcgen/Bcgen • 110935
| Product name | B-hCD80 mice |
|---|---|
| Catalog number | 110935 |
| Strain name | C57BL/6-Cd80tm1(CD80)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 941 (Human) |
| Aliases | B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1 |
Gene targeting strategy for B-hCD80 mice.
The exons 1-5 of mouse Cd80 gene that encode the extracellular domain were replaced by human CD80 exons 2-5 in B-hCD80 mice.
Strain specific analysis of CD80 gene expression in wild type (WT) mice and B-hCD80 mice by RT-PCR. Mouse Cd80 mRNA was detectable only in splenocytes of WT mice (+/+). Human CD80 mRNA was detectable only in homozygous B-hCD80 mice (H/H) but not in WT mice (+/+).
Strain specific CD80 expression analysis in homozygous B-hCD80 mice by flow cytometry. Peritoneal exudative macrophages (PEMs) were collected from wild type (WT) mice (+/+) and homozygous B-hCD80 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD80 antibody. Mouse CD80 was detectable in WT mice (+/+). Human CD80 was exclusively detectable in homozygous B-hCD80 mice (H/H) but not in WT mice (+/+).
Strain specific CD80 expression analysis in homozygous B-hCD80 mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD80 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD80 antibody. Mouse CD80 was detectable in WT mice (+/+). Human CD80 was exclusively detectable in homozygous B-hCD80 mice (H/H) but not in WT mice (+/+).
