C57BL/6-Il17ratm1(IL17RA)BcgenIl17rctm1(IL17RC)Bcgen/Bcgen • 120555
| Product name | B-hIL17RA/hIL17RC mice |
|---|---|
| Catalog number | 120555 |
| Strain name | C57BL/6-Il17ratm1(IL17RA)BcgenIl17rctm1(IL17RC)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 84818,23765 (Human) |
| Aliases | CANDF9; IL17RL; IL17-RL; CD217; IL17R; IMD51; CANDF5; CDw217; IL-17RA; hIL-17R |
Gene targeting strategy for B-hIL17RA/hIL17RC mice. The exons 2-9 of mouse Il17ra gene that encode the extracellular domain were replaced by human IL17RA exons 2-9 in B-hIL17RA/hIL17RC mice. The Chimeric IL17RC CDS CDS that encodes human IL17RC signal peptide and extracellular domain, mouse Il17rc transmembrane and cytoplasmic domain was inserted after the 5’UTR of mouse IL17rc gene in B-hIL17RA/hIL17RC mice.
Strain specific IL17RA expression analysis in C57BL/6 wild-type and homozygous B-hIL17RA/hIL17RC mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 (+/+) and homozygous B-hIL17RA/hIL17RC mice (H/H, H/H), and analyzed by flow cytometry with species-specific anti-IL17RA antibody. Mouse IL17RA was only detectable in wild-type mice. Human IL17RA were only detectable in macrophages of spleen from homozygous B-hIL17RA/hIL17RC mice .
Strain specific IL17RA expression analysis in C57BL/6 wild-type and homozygous B-hIL17RA/hIL17RC mice by flow cytometry. Bone marrow cells were collected from wild-type C57BL/6 (+/+) and homozygous B-hIL17RA/hIL17RC mice (H/H, H/H), and analyzed by flow cytometry with species-specific anti-IL17RA antibody. Mouse IL17RA was only detectable in wild-type mice. Human IL17RA were only detectable in macrophages of bone marrow from homozygous mice.
Strain specific IL17RC expression analysis in C57BL/6 wild-type and homozygous B-hIL17RA/hIL17RC mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 (+/+) and homozygous B-hIL17RA/hIL17RC mice (H/H, H/H), and analyzed by flow cytometry with species-specific anti-IL17RC antibody. Mouse IL17RC and human IL17RC were detectable in DCs, monocytes, macrophages and neutrophils from wild-type and homozygous mice, as anti-mouse IL17RC and anti-human IL17RC antibody were both cross-reactive between human IL17RC and mouse IL17RC.
Strain specific IL17RC expression analysis in C57BL/6 wild-type and homozygous B-hIL17RA/hIL17RC mice by flow cytometry. Bone marrow cells were collected from wild-type C57BL/6 (+/+) and homozygous B-hIL17RA/hIL17RC mice (H/H, H/H), and analyzed by flow cytometry with species-specific anti-IL17RC antibody. Mouse IL17RC and human IL17RC were detectable in DCs, monocytes, macrophages and neutrophils from wild-type and homozygous mice, as anti-mouse IL17RC and anti-human IL17RC antibody were both cross-reactive between human IL17RC and mouse IL17RC.
Strain specific analysis of IL17RC mRNA expression in wild-type C57BL/6 mice and B-hIL17RA/IL17RC mice by RT-PCR. Colon RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hIL17RA/hIL17RC mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human IL17RC primers. Mouse Il17rc mRNA was detectable only in wild-type C57BL/6 mice. Human IL17RC mRNA was detectable only in homozygous B-hIL17RA/hIL17RC mice, but not in wild-type mice.
