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    B-CAG-tdTomato cKI mice

    C57BL/6-Gt(ROSA)26Sortm1(CAG-tdTomato)Bcgen/Bcgen • 110148

    B-CAG-tdTomato cKI mice

    Product nameB-CAG-tdTomato cKI mice
    Catalog number110148
    Strain nameC57BL/6-Gt(ROSA)26Sortm1(CAG-tdTomato)Bcgen/Bcgen
    Strain backgroundC57BL/6N
    Official symbolROSA26、tdTomato
    Chromosome6
    ApplicationThis CAG-tdTomatomodel is an efficient tool to study the tissue specification of Cre mice, whencross with the Cre mice.

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    • Description
    • Targeting strategy
    • Phenotypic analysis

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      发表文章

        Description

        ROSAS26 gene produces a long non-coding RNA (lncRNA) that is under the control of a constitutive promoter. This locus is a widely used site for the integration of transgenes and reporter constructs.

        Gene editing strategy
        A CAG-LSL-tdTomato-WPRE-pA cassette was placed between the exon 1 and exon 2 using C57BL/6 embryonic stem cells. This strain was maintained on a C57BL/6 genetic background.
        Phenotype Analysis

        E

        Experimental design : T cells were isolated from peripheral blood in the single positive mice and the double-positive mice. The proportion of lymphocyte subpopulation were analyzed by flow cytometry.

        Results: tdTomato+ cells were detected in the double-positive mice.

        Experimental design : T cells were isolated from spleen in single positive mice and the double-positive mice. The proportion of lymphocyte subpopulation were analyzed by flow cytometry.

        Results: tdTomato+ cells were detected in the double-positive mice.

        * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-CAG-tdTomato cKI mice] (Cat# 110148) was purchased from Biocytogen.