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    B-hBMP9 mice

    C57BL/6N-Gdf2tm1(GDF2)Bcgen/Bcgen • 112354

    B-hBMP9 mice

    Product nameB-hBMP9 mice
    Catalog number112354
    Strain nameC57BL/6N-Gdf2tm1(GDF2)Bcgen/Bcgen
    Strain backgroundC57BL/6N
    NCBI gene ID2658 (Human)
    AliasesBMP9; HHT5; BMP-9

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    • Phenotypic analysis

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        Description
        • BMP9 is a secreted protein and after binding to its receptors, SMAD family transcription factors were recruited and activated, leading to gene expression.
        • Homozygous mice expressed the human BMP9 and developed normally.
        • This model is useful in studying the role of BMP9 in liver fibrosis.
        mRNA expression analysis

        Strain specific analysis of BMP9 mRNA expression in wild-type C57BL/6N mice and homozygous B-hBMP9 mice by RT-PCR. Liver RNA were isolated from wild-type C57BL/6N mice (+/+) and homozygous B-hBMP9 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human BMP9 primers. Mouse Bmp9 mRNA was detectable only in wild-type C57BL/6N mice. Human BMP9 mRNA was detectable only in homozygous B-hBMP9 mice but not in wild-type mice.

        Protein expression analysis
        Western blot analysis of BMP9 protein expression in homozygous B-hBMP9 mice. Plasma and serum were collected from wild-type C57BL/6N mice (+/+) and homozygous B-hBMP9 mice (H/H), (male, 7-week-old, n=2) and then analyzed by western blot with anti-BMP9 antibody (Abcam, ab207318). 20 μg total proteins were loaded for western blotting analysis. The antibody used showed cross-recognition for mouse and human BMP9.
        * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hBMP9 mice] (Cat# 112354) was purchased from Biocytogen.