• 德晋贵宾厅

    B-hBTLA/hHVEM mice

    C57BL/6-Btlatm1(BTLA)Bcgen Tnfrsf14tm1(TNFRSF14)Bcgen/Bcgen • 121080

    B-hBTLA/hHVEM mice

    Product nameB-hBTLA/hHVEM mice
    Catalog number121080
    Strain nameC57BL/6-Btlatm1(BTLA)Bcgen Tnfrsf14tm1(TNFRSF14)Bcgen/Bcgen
    Strain backgroundC57BL/6
    NCBI gene ID8764,151888 (Human)
    AliasesTR2; ATAR; HVEA; HVEM; CD270; LIGHTR; BTLA1; CD272

    在此页面上

    • Phenotypic analysis
    • Efficacy

    海报

    查看全部

      发表文章

        mRNA expression analysis
        Strain specific analysis of BTLA and HVEM gene expression in wild type (WT) mice and B-hBTLA/hHVEM mice by RT-PCR.Mouse Btla and Hvem mRNA was detectable only in splenocytes of WT mice (+/+). Human BTLA and HVEM mRNA was detectable only in homozygous B-hBTLA/hHVEM mice (H/H;H/H) but not in WT mice (+/+).
        Protein expression analysis in B cells
        Strain specific BTLA expression analysis in homozygous B-hBTLA/hHVEM mice by flow cytometry.Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hBTLA/hHVEM mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-BTLA antibody. Mouse BTLA was detectable in WT mice (+/+). Human BTLA was exclusively detectable in homozygous B-hBTLA/hHVEM mice (H/H;H/H) but not in WT mice (+/+).
        Protein expression analysis in T cells
        Strain specific HVEM expression analysis in homozygous B-hBTLA/hHVEM mice by flow cytometry.Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hBTLA/hHVEM mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-HVEM antibody. Mouse HVEM was detectable in WT mice (+/+). Human HVEM was exclusively detectable in homozygous B-hBTLA/hHVEM mice (H/H;H/H) but not in WT mice (+/+).
        Tumor growth curve & Body weight changes›

        Subcutaneous homograft tumor growth of B-hHVEM MC38 cells. B-hHVEM MC38 cells (1x106) and wild-type MC38 cells (5x105) were subcutaneously implanted into homozygous B-hBTLA/hHVEM mice (female, 6-week-old, n=7). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean ± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hHVEM MC38 cells were able to form tumors in vivo and can be used for efficacy studies.

        Tumor growth curve of individual mice

        B-hHVEM MC38 tumor cells growth of individual mice. B-hHVEM MC38 cells (1x106) and wild-type MC38 cells (5x105) were subcutaneously implanted into homozygous B-hBTLA/hHVEM mice (female, 6-week-old, n=7). As shown in panel, B-hHVEM MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

        * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hBTLA/hHVEM mice] (Cat# 121080) was purchased from Biocytogen.