C57BL/6-Ccr4tm1(CCR4)Bcgen/Bcgen • 110098
| Product name | B-hCCR4 mice |
|---|---|
| Catalog number | 110098 |
| Strain name | C57BL/6-Ccr4tm1(CCR4)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 1233 (Human) |
| Aliases | CKR4; K5-5; CD194; CMKBR4; ChemR13; CC-CKR-4; HGCN:14099 |
Gene targeting strategy for B-hCCR4 mice. The exon 2 of mouse Ccr4 gene that encodes the full-length protein was replaced by human CCR4 exon 2 in B-hCCR4 mice.
Strain specific analysis of CCR4 gene expression in WT and hCCR4 mice by RT-PCR. Mouse Ccr4 mRNA was detectable only in splenocytes of wild-type (+/+) mice. Human CCR4 mRNA was detectable only in H/H, but not in +/+ mice.
Strain specific CCR4 expression analysis in homozygous B-hCCR4 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCCR4 (H/H) mice, and analyzed by flow cytometry with species-specific anti-CCR4 antibody. Mouse CCR4 was detectable in WT mice. Human CCR4 was exclusively detectable in homozygous B-hCCR4 but not WT mice.
Strain specific CCR4 expression analysis in homozygous B-hCCR4 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCCR4 (H/H) mice, and analyzed by flow cytometry with species-specific anti-CCR4 antibody. Mouse CCR4 was detectable in WT mice. Human CCR4 was exclusively detectable in homozygous B-hCCR4 but not WT mice.
