C57BL/6-Cd3etm2(CD3E)Bcgen Cd38tm3(CD38)Bcgen/Bcgen • 111873
| Product name | B-hCD3E/hCD38 mice |
|---|---|
| Catalog number | 111873 |
| Strain name | C57BL/6-Cd3etm2(CD3E)Bcgen Cd38tm3(CD38)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 916,952 (Human) |
| Aliases | T3E; TCRE; IMD18; CD3epsilon; ADPRC1; cADPR1; ADPRC 1 |
Gene targeting strategy for B-hCD3E/hCD38 mice.
The exons 2-6 of mouse Cd3e gene that encode the extracellular domain were replaced by human CD3E exons 2-7 in B-hCD3E/hCD38 mice. The CDS of human CD38 encoding the extracellular domain was inserted following partial exon 2 of mouse Cd38 in B-hCD3E/hCD38 mice.
Strain specific CD3E expression analysis in homozygous B-hCD3E/hCD38 mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3E/hCD38 mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD3ε antibody. Mouse CD3E was detectable in WT mice (+/+). Human CD3E was exclusively detectable in homozygous B-hCD3E/hCD38 mice (H/H;H/H) but not in WT mice (+/+).
Strain specific CD38 expression analysis in homozygous B-hCD3E/hCD38 mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3E/hCD38 mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD38 antibody. Mouse CD38 was detectable in WT mice (+/+). Human CD38 was exclusively detectable in homozygous B-hCD3E/hCD38 mice (H/H;H/H) but not in WT mice (+/+).
