C57BL/6-Ednratm1(ETAR)Bcgen /Bcgen • 110108
| Product name | B-hETAR mice |
|---|---|
| Catalog number | 110108 |
| Strain name | C57BL/6-Ednratm1(ETAR)Bcgen /Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 1909 (Human) |
| Aliases | ETA; ET-A; ETAR; ETRA; MFDA; ETA-R; hET-AR |
Gene targeting strategy for B-hETAR mice. The human ETAR CDS was insert into mouse Etar exon 2 in B-hETAR mice.
Strain specific analysis of ETAR gene expression in B-hETAR mice by RT-PCR. Mouse Etar mRNA was detectable in lung of wild-type (+/+) mice. Human ETAR mRNA was detectable only in homozygous B-hETAR mice (H/H) but not in wild-type mice.
Strain specific ETAR expression analysis in homozygous B-hETAR mice by flow cytometry. Monocytes and macrophages of bone marrow were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hETAR mice (H/H), and analyzed by flow cytometry with species-specific anti-ETAR antibody. Mouse Etar was detectable in wild-type mice. This anti-mouse ETAR antibody also cross reacts with human ETAR. Taken together with RT results, B-hETAR mice successfully express human ETAR protein.
