C57BL/6-Grptm1(GRP)Bcgen/Bcgen • 111902
| Product name | B-hGRP mice |
|---|---|
| Catalog number | 111902 |
| Strain name | C57BL/6-Grptm1(GRP)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 2922 (Human) |
| Aliases | BN; GRP-10; proGRP; preproGRP |
Gene targeting strategy for B-hGRP mice. The exons 1-3 of mouse Grp gene that encode the full-length protein were replaced by human GRP exons 1-3 in B-hGRP mice.
Strain specific analysis of GRP gene expression in wild-type C57BL/6 mice and B-hGRP mice by RT-PCR. Mouse Grp mRNA was detectable in brain of wild-type C57BL/6 mice (+/+). Human GRP mRNA was detectable only in homozygous B-hGRP but not in wild-type mice.
Strain specific GRP expression analysis in homozygous B-hGRP mice by western blot. Brain tissue was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hGRP mice (H/H), and analyzed by western blot with anti-GRP antibody. GRP was detectable in wild-type mice and homozygous B-hGRP mice, as the antibody is cross-reactive between human and mouse.
