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B-hIBSP mice

C57BL/6N-Ibsptm1(IBSP)Bcgen/Bcgen • 110930

B-hIBSP mice

Product nameB-hIBSP mice
Catalog number110930
Strain nameC57BL/6N-Ibsptm1(IBSP)Bcgen/Bcgen
Strain backgroundC57BL/6N
NCBI gene ID3381 (Human)
AliasesBSP; BNSP; SP-II; BSP II; BSP-II

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  • Targeting strategy
  • Phenotypic analysis

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    发表文章

      Targeting strategy

      Gene targeting strategy for B-hIBSP mice. The exons 2~7 of mouse Ibsp gene that encodes the full-length protein was replaced by human IBSP exons 2~7 in B-hIBSP mice.

      Protein expression analysis

      Strain specific IBSP expression analysis in homozygous B-hIBSP mice by ELISA. Serum was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hIBSP mice (H/H) and analyzed by ELISA with IBSP ELISA kit. Mouse IBSP was detectable in wild-type mice and homozygous B-hIBSP mice as the kit has partly cross reactions with human and mouse IBSP (A). Human IBSP was exclusively detectable in homozygous B-hIBSP mice but not in wild-type mice (B).

      mRNA expression analysis
      Strain specific analysis of IBSP mRNA expression in wild-type C57BL/6 mice and B-hIBSP mice by RT-PCR. Bone marrow RNA was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hIBSP mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human IBSP primers. Mouse Ibsp mRNA was detectable only in wild-type C57BL/6 mice. Human IBSP mRNA was detectable only in homozygous B-hIBSP mice but not in wild-type mice.
      * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hIBSP mice] (Cat# 110930) was purchased from Biocytogen.