C57BL/6-Il27tm1(IL27)Bcgen/Bcgen • 110763
| Product name | B-hIL27 mice |
|---|---|
| Catalog number | 110763 |
| Strain name | C57BL/6-Il27tm1(IL27)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 246778 (Human) |
| Aliases | p28; IL30; IL-27; IL27A; IL-27A; IL27p28 |
Gene targeting strategy for B-hIL27 mice. The mouse Il27 gene was replaced by full coding region gene sequence of human IL27 in B-hIL27 mice.
Strain specific analysis of IL27 gene expression in wild-type mice and B-hIL27 mice by RT-PCR. Mouse Il27 was detectable in the BMDCs of wild-type mice (+/+). Human IL27 was only detectable in homozygous B-hIL27 mice but not in wild-type mice.
Strain specific IL27 expression analysis in homozygous B-hIL27 mice by ELISA. BMDC culture supernatant was collected from wild-type mice (+/+) and homozygous B-hIL27 mice (H/H) stimulated with LPS (1 μg/mL) for 24h, and analyzed by ELISA with species-specific IL27 ELISA kit. Mouse IL27 was exclusively detectable in wild-type mice. Human IL27 was detectable in homozygous B-hIL27 mice but not in wild-type mice.
