C57BL/6-Itgb1tm1(ITGB1)Bcgen/Bcgen • 110901
| Product name | B-hITGB1 mice |
|---|---|
| Catalog number | 110901 |
| Strain name | C57BL/6-Itgb1tm1(ITGB1)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 3688 (Human) |
| Aliases | CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA |
Gene targeting strategy for B-hITGB1 mice. The exons 2-15 of mouse Itgb1 gene were replaced by human ITGB1 exons 2-15 in B-hITGB1 mice.
Strain specific analysis of ITGB1 gene expression in wild-type mice and B-hITGB1 mice by RT-PCR. Mouse Itgb1 mRNA was detectable in splenocytes of wild-type mice (+/+). Human ITGB1 mRNA was detectable only in homozygous B-hITGB1 but not in wild-type mice.
Strain specific ITGB1 expression analysis in homozygous B-hITGB1 mice by flow cytometry. Splenocytes were collected from wild-type mice (+/+) and homozygous B-hITGB1 mice (H/H) analyzed by flow cytometry with species-specific anti-ITGB1 antibody. Mouse ITGB1 was detectable in WT mice. Human ITGB1 were exclusively detectable in homozygous B-hITGB1 mice (H/H).
