C57BL/6JNifdc-Sema7atm1(SEMA7A)Bcgen/Bcgen • 112517
| Product name | B-hSEMA7A mice |
|---|---|
| Catalog number | 112517 |
| Strain name | C57BL/6JNifdc-Sema7atm1(SEMA7A)Bcgen/Bcgen |
| Strain background | C57BL/6JNifdc |
| NCBI gene ID | 8482 (Human) |
| Aliases | JMH; CD108; SEMAL; CDw108; PFIC11; SEMAK1; H-Sema-L; H-SEMA-K1 |
Gene targeting strategy for B-hSEMA7A mice. All exons 1~14 of mouse Sema7a gene that encode the full-length protein were replaced by human SEMA7A exons 1~14 in B-hSEMA7A mice.
Western blot analysis of SEMA7A protein expression in homozygous B-hSEMA7A mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA7A mice (H/H), and then analyzed by western blot with species-specific anti-SEMA7A antibody. 40 μg total proteins were loaded for western blotting analysis. SEMA7A was detected in brain, testis, lung and colon.
Strain specific analysis of SEMA7A mRNA expression in wild-type C57BL/6 mice and B-hSEMA7A mice by RT-PCR. Brain RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA7A mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human SEMA7A primers. Mouse Sema7a mRNA was detectable only in wild-type C57BL/6 mice. Human SEMA7A mRNA was detectable only in homozygous B-hSEMA7A mice but not in wild-type mice.
