Targeting strategy
Gene targeting strategy for B-hTEK mice. The exons 2-14 of mouse Tek gene were replaced by human TEK exons 2-14 in B-hTEK mice.
mRNA expression analysis
Strain specific analysis of TEK gene expression in wild-type mice and B-hTEK mice by RT-PCR.Mouse Tek mRNA was detectable in the lung of wild-type mice (+/+). Human TEK mRNA was detectable only in homozygous B-hTEK but not in wild-type mice.
Protein expression analysis
Strain specific TEK expression analysis in homozygous B-hTEK mice by flow cytometry.Lung was collected from wild-type mice (+/+) and homozygous B-hTEK mice (H/H), and analyzed by flow cytometry with anti-TEK antibody. Mouse TEK was only detectable in wild-type mice. Human TEK was detectable in both homozygous B-hTEK mice and wild-type mice because of the cross-action antibody.
* When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hTEK mice] (Cat# 111106) was purchased from Biocytogen.
